Dissecting self-renewal in stem cells with RNA interference

Natalia Ivanova (), Radu Dobrin, Rong Lu, Iulia Kotenko, John Levorse, Christina DeCoste, Xenia Schafer, Yi Lun and Ihor R. Lemischka ()
Additional contact information
Natalia Ivanova: Princeton University
Radu Dobrin: Princeton University
Rong Lu: Princeton University
Iulia Kotenko: Princeton University
John Levorse: Princeton University
Christina DeCoste: Princeton University
Xenia Schafer: Princeton University
Yi Lun: Princeton University
Ihor R. Lemischka: Princeton University

Nature, 2006, vol. 442, issue 7102, 533-538

Abstract: Abstract We present an integrated approach to identify genetic mechanisms that control self-renewal in mouse embryonic stem cells. We use short hairpin RNA (shRNA) loss-of-function techniques to downregulate a set of gene products whose expression patterns suggest self-renewal regulatory functions. We focus on transcriptional regulators and identify seven genes for which shRNA-mediated depletion negatively affects self-renewal, including four genes with previously unrecognized roles in self-renewal. Perturbations of these gene products are combined with dynamic, global analyses of gene expression. Our studies suggest specific biological roles for these molecules and reveal the complexity of cell fate regulation in embryonic stem cells.

Date: 2006
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DOI: 10.1038/nature04915

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