Proteolytic turnover of the Gal4 transcription factor is not required for function in vivo

Kip Nalley, Stephen Albert Johnston and Thomas Kodadek ()
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Kip Nalley: University of Texas Southwestern Medical Center
Stephen Albert Johnston: University of Texas Southwestern Medical Center
Thomas Kodadek: University of Texas Southwestern Medical Center

Nature, 2006, vol. 442, issue 7106, 1054-1057

Abstract: Seen the Movie Yet? Salivary glands of Drosophila larvae contain giant polytene chromosomes whose characteristic banded structure is readily visible by light microscopy. These chromosomes are usually visualized by breaking the nuclei, then 'spreading' the chromosomes in two dimensions. But using two-photon laser-scanning microscopy, they can now be examined in living salivary gland tissue in three dimensions and in real time. The above image is a 3D-reconstructed view of polytene nuclei where DNA is stained red and transcription factor HSF is shown green, before (top) and after heat shock. This technique reveals the dynamics of the interaction between DNA and a model transcription factor, as illustrated in a movie in Supplementary Information. Nalley et al., using different methods, draw similar conclusions about the dynamics of transcription factor Gal4.

Date: 2006
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DOI: 10.1038/nature05067

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