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Stoichiometry and turnover in single, functioning membrane protein complexes

Mark C. Leake, Jennifer H. Chandler, George H. Wadhams, Fan Bai, Richard M. Berry and Judith P. Armitage ()
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Mark C. Leake: University of Oxford
Jennifer H. Chandler: University of Oxford
George H. Wadhams: University of Oxford
Fan Bai: University of Oxford
Richard M. Berry: University of Oxford
Judith P. Armitage: University of Oxford

Nature, 2006, vol. 443, issue 7109, 355-358

Abstract: The dynamic exchange of motor proteins in a functioning 45-nm rotary complex was observed in vivo and GFP–MotB stator proteins visualized in individual bacterial flagellar motors. Fluorescence recovery after photobleaching reveals that the ∼22 MotB motor proteins exchange rapidly with a pool of free protein while the motor is actively rotating.

Date: 2006
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DOI: 10.1038/nature05135

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