Watching hydrogen-bond dynamics in a β-turn by transient two-dimensional infrared spectroscopy

Christoph Kolano (), Jan Helbing, Mariusz Kozinski, Wolfram Sander and Peter Hamm ()
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Christoph Kolano: Physikalisch–Chemisches Institut, Universität Zürich
Jan Helbing: Physikalisch–Chemisches Institut, Universität Zürich
Mariusz Kozinski: Physikalisch–Chemisches Institut, Universität Zürich
Wolfram Sander: Ruhr–Universität Bochum
Peter Hamm: Physikalisch–Chemisches Institut, Universität Zürich

Nature, 2006, vol. 444, issue 7118, 469-472

Abstract: Abstract X-ray crystallography and nuclear magnetic resonance measurements provide us with atomically resolved structures of an ever-growing number of biomolecules. These static structural snapshots are important to our understanding of biomolecular function, but real biomolecules are dynamic entities that often exploit conformational changes and transient molecular interactions to perform their tasks. Nuclear magnetic resonance methods can follow such structural changes, but only on millisecond timescales under non-equilibrium conditions. Time-resolved X-ray crystallography has recently been used to monitor the photodissociation of CO from myoglobin on a subnanosecond timescale1, yet remains challenging to apply more widely. In contrast, two-dimensional infrared spectroscopy, which maps vibrational coupling between molecular groups and hence their relative positions and orientations2,3,4,5,6,7,8,9,10,11, is now routinely used to study equilibrium processes on picosecond timescales. Here we show that the extension of this method into the non-equilibrium regime12,13 allows us to observe in real time in a short peptide the weakening of an intramolecular hydrogen bond and concomitant opening of a β-turn. We find that the rate of this process is two orders of magnitude faster than the ‘folding speed limit’ established for contact formation between protein side chains14.

Date: 2006
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DOI: 10.1038/nature05352

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