Selection and evolution of enzymes from a partially randomized non-catalytic scaffold

Burckhard Seelig and Jack W. Szostak ()
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Burckhard Seelig: Howard Hughes Medical Institute, and Center for Computational and Integrative Biology (CCIB), 7215 Simches Research Center, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA
Jack W. Szostak: Howard Hughes Medical Institute, and Center for Computational and Integrative Biology (CCIB), 7215 Simches Research Center, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA

Nature, 2007, vol. 448, issue 7155, 828-831

Abstract: New enzymatic activities can be evolved de novo (that is, without the need for prior mechanistic information) by using mRNA-display. Functional proteins were selected for from an in vitro translated protein library of high complexity and it was possible to isolate novel RNA ligases that exhibited rate enhancements of more than two million-fold.

Date: 2007
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DOI: 10.1038/nature06032

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