Snapshots of nuclear pore complexes in action captured by cryo-electron tomography

Martin Beck, Vladan Lučić, Friedrich Förster, Wolfgang Baumeister () and Ohad Medalia ()
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Martin Beck: Max Planck Institute of Biochemistry
Vladan Lučić: Max Planck Institute of Biochemistry
Friedrich Förster: Max Planck Institute of Biochemistry
Wolfgang Baumeister: Max Planck Institute of Biochemistry
Ohad Medalia: Max Planck Institute of Biochemistry

Nature, 2007, vol. 449, issue 7162, 611-615

Abstract: The structure of the nuclear pore complexes in intact nuclei is studied by cryoelectron tomography. The trajectory of cargo molecules was traced used gold-labelled proteins fused to a nuclear localization signal. Superimposition of multiple tomograms has yielded a map outlining the pathway of cargo molecules during import into the nucleus.

Date: 2007
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DOI: 10.1038/nature06170

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