Producing primate embryonic stem cells by somatic cell nuclear transfer

J. A. Byrne, D. A. Pedersen, L. L. Clepper, M. Nelson, W. G. Sanger, S. Gokhale, D. P. Wolf and S. M. Mitalipov ()
Additional contact information
J. A. Byrne: Oregon National Primate Research Center and,
D. A. Pedersen: Oregon National Primate Research Center and,
L. L. Clepper: Oregon National Primate Research Center and,
M. Nelson: Munroe-Meyer Institute, 985450 Nebraska Medical Center, Omaha, Nebraska 68198, USA
W. G. Sanger: Munroe-Meyer Institute, 985450 Nebraska Medical Center, Omaha, Nebraska 68198, USA
S. Gokhale: Munroe-Meyer Institute, 985450 Nebraska Medical Center, Omaha, Nebraska 68198, USA
D. P. Wolf: Oregon National Primate Research Center and,
S. M. Mitalipov: Oregon National Primate Research Center and,

Nature, 2007, vol. 450, issue 7169, 497-502

Abstract: Abstract Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos. DNA analysis confirmed that nuclear DNA was identical to donor somatic cells and that mitochondrial DNA originated from oocytes. Both cell lines exhibited normal ES cell morphology, expressed key stem-cell markers, were transcriptionally similar to control ES cells and differentiated into multiple cell types in vitro and in vivo. Our results represent successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrate proof-of-concept for therapeutic cloning in primates.

Date: 2007
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/nature06357 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:450:y:2007:i:7169:d:10.1038_nature06357

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature06357

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().