Genotyping of Rhesus SCNT pluripotent stem cell lines

David S. Cram (), Bi Song () and Alan O. Trounson ()
Additional contact information
David S. Cram: Monash Immunology and Stem Cell Laboratories, Monash University
Bi Song: Monash Immunology and Stem Cell Laboratories, Monash University
Alan O. Trounson: Monash Immunology and Stem Cell Laboratories, Monash University

Nature, 2007, vol. 450, issue 7169, E12-E14

Abstract: Embryonic stem cells Techniques for reprogramming adult cells are much sought after for creating cells to genetically match those of patients. Such embryonic stem cells might be used therapeutically without immune rejection. One reprogramming method is somatic cell nuclear transfer, where an adult cell nucleus is inserted into an oocyte that has its own nucleus removed. An early (blastocyst) stage embryo is induced to form, from which embryonic stem cells are teased out and cultured. Previously, this was possible only in mice. Now Byrne et al. have succeeded with the technique using primate adult fibroblasts as the start point. They generated two embryonic stem cell lines from 304 oocytes taken from 14 rhesus monkeys. This success suggests that this approach might be suitable for generating patient-derived embryonic stem cells. A validation paper accompanies this package on the Nature web site. The colorized cover image shows an egg just prior to enucleation. The egg is held by a holding pipette and to the right, a sharp enucleation pipette points towards the nucleus.

Date: 2007
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature06456 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:450:y:2007:i:7169:d:10.1038_nature06456

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature06456

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().