High-resolution mapping of meiotic crossovers and non-crossovers in yeast

Eugenio Mancera, Richard Bourgon, Alessandro Brozzi, Wolfgang Huber and Lars M. Steinmetz ()
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Eugenio Mancera: European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Richard Bourgon: European Molecular Biology Laboratory, European Bioinformatics Institute
Alessandro Brozzi: European Molecular Biology Laboratory, European Bioinformatics Institute
Wolfgang Huber: European Molecular Biology Laboratory, European Bioinformatics Institute
Lars M. Steinmetz: European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany

Nature, 2008, vol. 454, issue 7203, 479-485

Abstract: Abstract Meiotic recombination has a central role in the evolution of sexually reproducing organisms. The two recombination outcomes, crossover and non-crossover, increase genetic diversity, but have the potential to homogenize alleles by gene conversion. Whereas crossover rates vary considerably across the genome, non-crossovers and gene conversions have only been identified in a handful of loci. To examine recombination genome wide and at high spatial resolution, we generated maps of crossovers, crossover-associated gene conversion and non-crossover gene conversion using dense genetic marker data collected from all four products of fifty-six yeast (Saccharomyces cerevisiae) meioses. Our maps reveal differences in the distributions of crossovers and non-crossovers, showing more regions where either crossovers or non-crossovers are favoured than expected by chance. Furthermore, we detect evidence for interference between crossovers and non-crossovers, a phenomenon previously only known to occur between crossovers. Up to 1% of the genome of each meiotic product is subject to gene conversion in a single meiosis, with detectable bias towards GC nucleotides. To our knowledge the maps represent the first high-resolution, genome-wide characterization of the multiple outcomes of recombination in any organism. In addition, because non-crossover hotspots create holes of reduced linkage within haplotype blocks, our results stress the need to incorporate non-crossovers into genetic linkage analysis.

Date: 2008
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DOI: 10.1038/nature07135

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