Does Rft1 flip an N-glycan lipid precursor?

Christian G. Frank (), Sumana Sanyal (), Jeffrey S. Rush, Charles J. Waechter and Anant K. Menon ()
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Christian G. Frank: Weill Cornell Medical College
Sumana Sanyal: Weill Cornell Medical College
Jeffrey S. Rush: University of Kentucky College of Medicine
Charles J. Waechter: University of Kentucky College of Medicine
Anant K. Menon: Weill Cornell Medical College

Nature, 2008, vol. 454, issue 7204, E3-E4

Abstract: Abstract Arising from: J. Helenius et al. Nature 415, 447–450 (2002)10.1038/415447a ; Helenius et al. reply Protein N-glycosylation requires flipping of the glycolipid Man5GlcNAc2-diphosphate dolichol (Man5GlcNAc2-PP-Dol) across the endoplasmic reticulum (ER)1,2,3. Helenius et al.4 report genetic evidence suggesting that Rft1, an essential ER membrane protein in yeast, is required directly to translocate Man5GlcNAc2-PP-Dol. We now show that a specific ER protein(s), but not Rft1, is required to flip Man5GlcNAc2-PP-Dol in reconstituted vesicles. Rft1 may have a critical accessory role in translocating Man5GlcNAc2-PP-Dol in vivo, but the Man5GlcNAc2-PP-Dol flippase itself remains to be identified.

Date: 2008
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DOI: 10.1038/nature07165

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