The pathogen protein EspFU hijacks actin polymerization using mimicry and multivalency

Nathan A. Sallee, Gonzalo M. Rivera, John E. Dueber, Dan Vasilescu, R. Dyche Mullins, Bruce J. Mayer and Wendell A. Lim ()
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Nathan A. Sallee: Graduate Program in Chemistry and Chemical Biology and,
Gonzalo M. Rivera: Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
John E. Dueber: University of California, San Francisco, 600 16th Street, San Francisco, California 94158, USA
Dan Vasilescu: Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
R. Dyche Mullins: University of California, San Francisco, 600 16th Street, San Francisco, California 94158, USA
Bruce J. Mayer: Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Wendell A. Lim: University of California, San Francisco, 600 16th Street, San Francisco, California 94158, USA

Nature, 2008, vol. 454, issue 7207, 1005-1008

Abstract: Abstract Enterohaemorrhagic Escherichia coli attaches to the intestine through actin pedestals that are formed when the bacterium injects its protein EspFU (also known as TccP) into host cells1. EspFU potently activates the host WASP (Wiskott–Aldrich syndrome protein) family of actin-nucleating factors, which are normally activated by the GTPase CDC42, among other signalling molecules. Apart from its amino-terminal type III secretion signal, EspFU consists of five-and-a-half 47-amino-acid repeats. Here we show that a 17-residue motif within this EspFU repeat is sufficient for interaction with N-WASP (also known as WASL). Unlike most pathogen proteins that interface with the cytoskeletal machinery, this motif does not mimic natural upstream activators: instead of mimicking an activated state of CDC42, EspFU mimics an autoinhibitory element found within N-WASP. Thus, EspFU activates N-WASP by competitively disrupting the autoinhibited state. By mimicking an internal regulatory element and not the natural activator, EspFU selectively activates only a precise subset of CDC42-activated processes. Although one repeat is able to stimulate actin polymerization, we show that multiple-repeat fragments have notably increased potency. The activities of these EspFU fragments correlate with their ability to coordinate activation of at least two N-WASP proteins. Thus, this pathogen has used a simple autoinhibitory fragment as a component to build a highly effective actin polymerization machine.

Date: 2008
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DOI: 10.1038/nature07170

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