Identification of Holliday junction resolvases from humans and yeast

Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel and Stephen C. West ()
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Stephen C. Y. Ip: Genetic Recombination Laboratory,
Ulrich Rass: Genetic Recombination Laboratory,
Miguel G. Blanco: Genetic Recombination Laboratory,
Helen R. Flynn: Protein Analysis and Proteomics Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
J. Mark Skehel: Protein Analysis and Proteomics Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
Stephen C. West: Genetic Recombination Laboratory,

Nature, 2008, vol. 456, issue 7220, 357-361

Abstract: Abstract Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Here we identify nucleases from Saccharomyces cerevisiae and human cells that promote Holliday junction resolution, in a manner analogous to that shown by the Escherichia coli Holliday junction resolvase RuvC. The human Holliday junction resolvase, GEN1, and its yeast orthologue, Yen1, were independently identified using two distinct experimental approaches: GEN1 was identified by mass spectrometry following extensive fractionation of HeLa cell-free extracts, whereas Yen1 was detected by screening a yeast gene fusion library for nucleases capable of Holliday junction resolution. The eukaryotic Holliday junction resolvases represent a new subclass of the Rad2/XPG family of nucleases. Recombinant GEN1 and Yen1 resolve Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated.

Date: 2008
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DOI: 10.1038/nature07470

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