Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Keisuke Kaji (), Katherine Norrby, Agnieszka Paca, Maria Mileikovsky, Paria Mohseni and Knut Woltjen
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Keisuke Kaji: MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh
Katherine Norrby: MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh
Agnieszka Paca: MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh
Maria Mileikovsky: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
Paria Mohseni: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
Knut Woltjen: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada

Nature, 2009, vol. 458, issue 7239, 771-775

Abstract: Virus-free iPS cells The discovery that non-germline adult cells can be reprogrammed to become pluripotent, able to differentiate into any cell type, opened up exciting possibilities. Reprogrammed cells — called induced pluripotent stem (iPS) cells — should have great potential in regenerative medicine, but most current methods of producing them involve viral gene delivery that could cause abnormalities in the induced cells. Two groups in this issue report on a collaboration that has succeeded in producing pluripotency in human cells without using viral vectors. Stable iPS cells were produced in both human and mouse fibroblasts using virus-derived 2A peptide sequences to create a multicistronic vector incorporating the reprogramming factors, delivered to the cell by the piggyBac transposon vector. The 2A-linked reprogramming factors, not required in the established iPS cell lines, were then removed.

Date: 2009
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DOI: 10.1038/nature07864

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