Cai et al. reply

Chen-Leng Cai, Jody C. Martin, Yunfu Sun, Li Cui, Lianchun Wang, Kunfu Ouyang, Lei Yang, Lei Bu, Xingqun Liang, Xiaoxue Zhang, William B. Stallcup, Christopher P. Denton, Andrew McCulloch, Ju Chen and Sylvia M. Evans
Additional contact information
Chen-Leng Cai: * Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
Jody C. Martin: University of California, San Diego, La Jolla, California 92093, USA
Yunfu Sun: * Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
Li Cui: * Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
Lianchun Wang: Complex Carbohydrate Research Centre, University of Georgia
Kunfu Ouyang: University of California, San Diego, La Jolla, California 92093, USA
Lei Yang: * Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
Lei Bu: * Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
Xingqun Liang: University of California, San Diego, La Jolla, California 92093, USA
Xiaoxue Zhang: * Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
William B. Stallcup: Developmental Neurobiology Programme, The Burnham Institute
Christopher P. Denton: Centre for Rheumatology, Royal Free and University College Medical School
Andrew McCulloch: University of California, San Diego, La Jolla, California 92093, USA
Ju Chen: University of California, San Diego, La Jolla, California 92093, USA
Sylvia M. Evans: * Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu

Nature, 2009, vol. 458, issue 7240, E9-E10

Abstract: Abstract Replying to: V. M. Christoffels et al. Nature 458, 10.1038/nature07916 (2009) We recognized the importance of confirming data from Cre lineage studies with an alternative approach, and thus used a fluorescent dye to lineage-label epicardial cells. This approach confirmed that epicardial cells contributed to myocytes within the heart1. In our experiments, we never observed Tbx18 mRNA within ventricles at embryonic day E10.5 to E11.5, although our RNA signal in epicardium at these stages is at least as strong as that observed by Christoffels et al.2. We note a discrepancy between amount of RNA staining (broadly throughout the middle of the ventricle) and protein staining (much more restricted) that Christoffels et al.2 observe at early stages. Reasons for discrepancies between our RNA in situ data and that of Christoffels et al. are unclear. Christoffels et al.2 detect Tbx18 intronic sequences by RT–PCR of E11.5 IVS, but do not provide data to demonstrate mature Tbx18 mRNA. Notably, we did observe active expression of Tbx18 in non-myocytes after E11.5 (ref. 1).

Date: 2009
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