Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

Toshiro Sato, Robert G. Vries, Hugo J. Snippert, Marc van de Wetering, Nick Barker, Daniel E. Stange, Johan H. van Es, Arie Abo, Pekka Kujala, Peter J. Peters and Hans Clevers ()
Additional contact information
Toshiro Sato: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Robert G. Vries: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Hugo J. Snippert: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Marc van de Wetering: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Nick Barker: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Daniel E. Stange: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Johan H. van Es: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Arie Abo: Nuvelo, Inc., 201 Industrial Road, Suite 310, San Carlos, California 94070-6211, USA
Pekka Kujala: The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
Peter J. Peters: The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
Hans Clevers: Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands

Nature, 2009, vol. 459, issue 7244, 262-265

Abstract: Niche-free stem-cell organization Sato et al. present a method for establishing long-term culture conditions whereby single stem cells or single crypt structures isolated from intestinal crypts can generate organoids with all the differentiated cell types and architecture of intestinal crypts present in adult mammals. The stem cells they used to start the cultures were marked by Lrg5+, a protein that in an earlier study had been shown to mark six cycling stem cells that renew the rapidly self-renewing tissue of the intestine. They conclude that intestinal crypt-villus units are self-organizing structures, which can be built from a single stem cell in the absence of a cellular niche.

Date: 2009
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DOI: 10.1038/nature07935

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