Precise genome modification in the crop species Zea mays using zinc-finger nucleases

Vipula K. Shukla (), Yannick Doyon, Jeffrey C. Miller, Russell C. DeKelver, Erica A. Moehle, Sarah E. Worden, Jon C. Mitchell, Nicole L. Arnold, Sunita Gopalan, Xiangdong Meng, Vivian M. Choi, Jeremy M. Rock, Ying-Ying Wu, George E. Katibah, Gao Zhifang, David McCaskill, Matthew A. Simpson, Beth Blakeslee, Scott A. Greenwalt, Holly J. Butler, Sarah J. Hinkley, Lei Zhang, Edward J. Rebar, Philip D. Gregory and Fyodor D. Urnov
Additional contact information
Vipula K. Shukla: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Yannick Doyon: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Jeffrey C. Miller: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Russell C. DeKelver: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Erica A. Moehle: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Sarah E. Worden: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Jon C. Mitchell: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Nicole L. Arnold: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Sunita Gopalan: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Xiangdong Meng: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Vivian M. Choi: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Jeremy M. Rock: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Ying-Ying Wu: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
George E. Katibah: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Gao Zhifang: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
David McCaskill: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Matthew A. Simpson: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Beth Blakeslee: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Scott A. Greenwalt: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Holly J. Butler: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA
Sarah J. Hinkley: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Lei Zhang: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Edward J. Rebar: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Philip D. Gregory: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA
Fyodor D. Urnov: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA

Nature, 2009, vol. 459, issue 7245, 437-441

Abstract: On target for new plant genes The scope for improvement of yield and disease resistance of crop plants by genetic engineering has been limited by the lack of an efficient method for targeted gene modification. Zinc-finger protein technology looks set to fill the gap. This relies on the use of designed zinc-finger nucleases, artificial chimaeric proteins that exploit the natural recognition mechanism of cellular DNA repair machinery, to make sequence-specific double-stranded DNA breaks at a target locus. In this issue two groups report the successful application of this emerging technique. Shukla et al. modify the maize gene IPK1, thereby introducing both herbicide tolerance and modified phytate metabolism into this important crop plant. Townsend et al. target the SuR loci in tobacco plants, conferring resistance to imidazolinone and sulphonylurea herbicides. The method achieves a high frequency of gene targeting and should be suitable for the routine modification of endogenous plant genes.

Date: 2009
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DOI: 10.1038/nature07992

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