Distinctive chromatin in human sperm packages genes for embryo development

Saher Sue Hammoud, David A. Nix, Haiying Zhang, Jahnvi Purwar, Douglas T. Carrell () and Bradley R. Cairns ()
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Saher Sue Hammoud: Howard Hughes Medical Institute, and Huntsman Cancer Institute
David A. Nix: Research Informatics and Bioinformatics Core Facility, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
Haiying Zhang: Howard Hughes Medical Institute, and Huntsman Cancer Institute
Jahnvi Purwar: Howard Hughes Medical Institute, and Huntsman Cancer Institute
Douglas T. Carrell: IVF and Andrology Laboratories, Obstetrics and Gynecology, and Physiology
Bradley R. Cairns: Howard Hughes Medical Institute, and Huntsman Cancer Institute

Nature, 2009, vol. 460, issue 7254, 473-478

Abstract: Abstract Because nucleosomes are widely replaced by protamine in mature human sperm, the epigenetic contributions of sperm chromatin to embryo development have been considered highly limited. Here we show that the retained nucleosomes are significantly enriched at loci of developmental importance, including imprinted gene clusters, microRNA clusters, HOX gene clusters, and the promoters of stand-alone developmental transcription and signalling factors. Notably, histone modifications localize to particular developmental loci. Dimethylated lysine 4 on histone H3 (H3K4me2) is enriched at certain developmental promoters, whereas large blocks of H3K4me3 localize to a subset of developmental promoters, regions in HOX clusters, certain noncoding RNAs, and generally to paternally expressed imprinted loci, but not paternally repressed loci. Notably, trimethylated H3K27 (H3K27me3) is significantly enriched at developmental promoters that are repressed in early embryos, including many bivalent (H3K4me3/H3K27me3) promoters in embryonic stem cells. Furthermore, developmental promoters are generally DNA hypomethylated in sperm, but acquire methylation during differentiation. Taken together, epigenetic marking in sperm is extensive, and correlated with developmental regulators.

Date: 2009
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DOI: 10.1038/nature08162

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