Crystal structure of the ATP-gated P2X4 ion channel in the closed state

Toshimitsu Kawate, Jennifer Carlisle Michel, William T. Birdsong and Eric Gouaux ()
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Toshimitsu Kawate: Vollum Institute,
Jennifer Carlisle Michel: Vollum Institute,
William T. Birdsong: Vollum Institute,
Eric Gouaux: Vollum Institute,

Nature, 2009, vol. 460, issue 7255, 592-598

Abstract: Abstract P2X receptors are cation-selective ion channels gated by extracellular ATP, and are implicated in diverse physiological processes, from synaptic transmission to inflammation to the sensing of taste and pain. Because P2X receptors are not related to other ion channel proteins of known structure, there is at present no molecular foundation for mechanisms of ligand-gating, allosteric modulation and ion permeation. Here we present crystal structures of the zebrafish P2X4 receptor in its closed, resting state. The chalice-shaped, trimeric receptor is knit together by subunit–subunit contacts implicated in ion channel gating and receptor assembly. Extracellular domains, rich in β-strands, have large acidic patches that may attract cations, through fenestrations, to vestibules near the ion channel. In the transmembrane pore, the ‘gate’ is defined by an ∼8 Å slab of protein. We define the location of three non-canonical, intersubunit ATP-binding sites, and suggest that ATP binding promotes subunit rearrangement and ion channel opening.

Date: 2009
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DOI: 10.1038/nature08198

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