miR-145 and miR-143 regulate smooth muscle cell fate and plasticity

Kimberly R. Cordes, Neil T. Sheehy, Mark P. White, Emily C. Berry, Sarah U. Morton, Alecia N. Muth, Ting-Hein Lee, Joseph M. Miano, Kathryn N. Ivey and Deepak Srivastava ()
Additional contact information
Kimberly R. Cordes: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
Neil T. Sheehy: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
Mark P. White: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
Emily C. Berry: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
Sarah U. Morton: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
Alecia N. Muth: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
Ting-Hein Lee: Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
Joseph M. Miano: Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
Kathryn N. Ivey: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
Deepak Srivastava: Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA

Nature, 2009, vol. 460, issue 7256, 705-710

Abstract: Abstract MicroRNAs (miRNAs) are regulators of myriad cellular events, but evidence for a single miRNA that can efficiently differentiate multipotent stem cells into a specific lineage or regulate direct reprogramming of cells into an alternative cell fate has been elusive. Here we show that miR-145 and miR-143 are co-transcribed in multipotent murine cardiac progenitors before becoming localized to smooth muscle cells, including neural crest stem-cell-derived vascular smooth muscle cells. miR-145 and miR-143 were direct transcriptional targets of serum response factor, myocardin and Nkx2-5 (NK2 transcription factor related, locus 5) and were downregulated in injured or atherosclerotic vessels containing proliferating, less differentiated smooth muscle cells. miR-145 was necessary for myocardin-induced reprogramming of adult fibroblasts into smooth muscle cells and sufficient to induce differentiation of multipotent neural crest stem cells into vascular smooth muscle. Furthermore, miR-145 and miR-143 cooperatively targeted a network of transcription factors, including Klf4 (Kruppel-like factor 4), myocardin and Elk-1 (ELK1, member of ETS oncogene family), to promote differentiation and repress proliferation of smooth muscle cells. These findings demonstrate that miR-145 can direct the smooth muscle fate and that miR-145 and miR-143 function to regulate the quiescent versus proliferative phenotype of smooth muscle cells.

Date: 2009
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DOI: 10.1038/nature08195

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