Mitochondrial gene replacement in primate offspring and embryonic stem cells

Masahito Tachibana, Michelle Sparman, Hathaitip Sritanaudomchai, Hong Ma, Lisa Clepper, Joy Woodward, Ying Li, Cathy Ramsey, Olena Kolotushkina and Shoukhrat Mitalipov ()
Additional contact information
Masahito Tachibana: Oregon National Primate Research Center,
Michelle Sparman: Oregon National Primate Research Center,
Hathaitip Sritanaudomchai: Oregon National Primate Research Center,
Hong Ma: Oregon National Primate Research Center,
Lisa Clepper: Oregon National Primate Research Center,
Joy Woodward: Oregon National Primate Research Center,
Ying Li: Oregon National Primate Research Center,
Cathy Ramsey: Oregon National Primate Research Center,
Olena Kolotushkina: Oregon National Primate Research Center,
Shoukhrat Mitalipov: Oregon National Primate Research Center,

Nature, 2009, vol. 461, issue 7262, 367-372

Abstract: Abstract Mitochondria are found in all eukaryotic cells and contain their own genome (mitochondrial DNA or mtDNA). Unlike the nuclear genome, which is derived from both the egg and sperm at fertilization, the mtDNA in the embryo is derived almost exclusively from the egg; that is, it is of maternal origin. Mutations in mtDNA contribute to a diverse range of currently incurable human diseases and disorders. To establish preclinical models for new therapeutic approaches, we demonstrate here that the mitochondrial genome can be efficiently replaced in mature non-human primate oocytes (Macaca mulatta) by spindle–chromosomal complex transfer from one egg to an enucleated, mitochondrial-replete egg. The reconstructed oocytes with the mitochondrial replacement were capable of supporting normal fertilization, embryo development and produced healthy offspring. Genetic analysis confirmed that nuclear DNA in the three infants born so far originated from the spindle donors whereas mtDNA came from the cytoplast donors. No contribution of spindle donor mtDNA was detected in offspring. Spindle replacement is shown here as an efficient protocol replacing the full complement of mitochondria in newly generated embryonic stem cell lines. This approach may offer a reproductive option to prevent mtDNA disease transmission in affected families.

Date: 2009
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DOI: 10.1038/nature08368

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