Mapping GFP structure evolution during proton transfer with femtosecond Raman spectroscopy

Chong Fang, Renee R. Frontiera, Rosalie Tran and Richard A. Mathies ()
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Chong Fang: University of California, Berkeley, California 94720, USA
Renee R. Frontiera: University of California, Berkeley, California 94720, USA
Rosalie Tran: University of California, Berkeley, California 94720, USA
Richard A. Mathies: University of California, Berkeley, California 94720, USA

Nature, 2009, vol. 462, issue 7270, 200-204

Abstract: Inside green fluorescent protein GFP, the green fluorescent protein from the jellyfish Aequorea victoria, is widely used in the life sciences as a gene expression marker because of its efficient bioluminescence. The atomistic details of the excited-state proton transfer that generates this bioluminescence are still not fully understood. Fang et al. have used femtosecond stimulated Raman spectroscopy of the GFP chromophore to obtain detailed time-resolved vibrational spectra that reveal how a low-frequency skeletal vibration poises the protein for the critical proton transfer process. As well as throwing light on a reaction central to countless biological studies, this method of making real-time observations of the structural changes of a molecule during a multidimensional chemical reaction should have broad application in the determination of reaction mechanisms.

Date: 2009
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DOI: 10.1038/nature08527

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