DNA nanomechanics allows direct digital detection of complementary DNA and microRNA targets

Sudhir Husale, Henrik H. J. Persson and Ozgur Sahin ()
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Sudhir Husale: The Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA
Henrik H. J. Persson: Stanford Genome Technology Center, 855 California Avenue, Palo Alto, California 94304, USA
Ozgur Sahin: The Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA

Nature, 2009, vol. 462, issue 7276, 1075-1078

Abstract: A hint of nucleic acid The ability to detect a specific DNA sequence at very low levels is invaluable in applications such as genetics and diagnostics. The best current technologies require amplification of the sample and/or labelling. In this study, a new method that avoids such sample preparation steps is described. It exploits a unique mechanical property of hybridized DNA and RNA molecules that serves as an intrinsic molecular label and can achieve detection with attomolar (10−18 M) level sensitivity in pure samples. To demonstrate a more physiological application, the hybridization method was used to classify tumours on the basis of detection of tumour-specific microRNAs.

Date: 2009
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DOI: 10.1038/nature08626

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