Systems survey of endocytosis by multiparametric image analysis

Claudio Collinet, Martin Stöter, Charles R. Bradshaw, Nikolay Samusik, Jochen C. Rink, Denise Kenski, Bianca Habermann, Frank Buchholz, Robert Henschel, Matthias S. Mueller, Wolfgang E. Nagel, Eugenio Fava, Yannis Kalaidzidis and Marino Zerial ()
Additional contact information
Claudio Collinet: Max Planck Institute for Molecular Cell Biology and Genetics,
Martin Stöter: High-Throughput Technology Development Studio, MPI-CBG, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Charles R. Bradshaw: Max Planck Institute for Molecular Cell Biology and Genetics,
Nikolay Samusik: Max Planck Institute for Molecular Cell Biology and Genetics,
Jochen C. Rink: Present addresses: University of Utah School of Medicine, 401 MREB, 20 North 1900 East, Salt Lake City, Utah 84132-3401, USA (J.C.R.); Sirna Therapeutics Inc., San Francisco, California 94158, USA (D.K.).
Denise Kenski: Present addresses: University of Utah School of Medicine, 401 MREB, 20 North 1900 East, Salt Lake City, Utah 84132-3401, USA (J.C.R.); Sirna Therapeutics Inc., San Francisco, California 94158, USA (D.K.).
Bianca Habermann: Max Planck Institute for Molecular Cell Biology and Genetics,
Frank Buchholz: Max Planck Institute for Molecular Cell Biology and Genetics,
Robert Henschel: Center for Information Services and High Performance Computing (ZIH), Dresden University of Technology
Matthias S. Mueller: Center for Information Services and High Performance Computing (ZIH), Dresden University of Technology
Wolfgang E. Nagel: Center for Information Services and High Performance Computing (ZIH), Dresden University of Technology
Eugenio Fava: High-Throughput Technology Development Studio, MPI-CBG, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Yannis Kalaidzidis: Max Planck Institute for Molecular Cell Biology and Genetics,
Marino Zerial: Max Planck Institute for Molecular Cell Biology and Genetics,

Nature, 2010, vol. 464, issue 7286, 243-249

Abstract: Abstract Endocytosis is a complex process fulfilling many cellular and developmental functions. Understanding how it is regulated and integrated with other cellular processes requires a comprehensive analysis of its molecular constituents and general design principles. Here, we developed a new strategy to phenotypically profile the human genome with respect to transferrin (TF) and epidermal growth factor (EGF) endocytosis by combining RNA interference, automated high-resolution confocal microscopy, quantitative multiparametric image analysis and high-performance computing. We identified several novel components of endocytic trafficking, including genes implicated in human diseases. We found that signalling pathways such as Wnt, integrin/cell adhesion, transforming growth factor (TGF)-β and Notch regulate the endocytic system, and identified new genes involved in cargo sorting to a subset of signalling endosomes. A systems analysis by Bayesian networks further showed that the number, size, concentration of cargo and intracellular position of endosomes are not determined randomly but are subject to specific regulation, thus uncovering novel properties of the endocytic system.

Date: 2010
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature08779 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:464:y:2010:i:7286:d:10.1038_nature08779

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature08779

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().