Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

Beate Neumann, Thomas Walter, Jean-Karim Hériché, Jutta Bulkescher, Holger Erfle, Christian Conrad, Phill Rogers, Ina Poser, Michael Held, Urban Liebel, Cihan Cetin, Frank Sieckmann, Gregoire Pau, Rolf Kabbe, Annelie Wünsche, Venkata Satagopam, Michael H. A. Schmitz, Catherine Chapuis, Daniel W. Gerlich, Reinhard Schneider, Roland Eils, Wolfgang Huber, Jan-Michael Peters, Anthony A. Hyman, Richard Durbin, Rainer Pepperkok and Jan Ellenberg ()
Additional contact information
Beate Neumann: MitoCheck Project Group,
Thomas Walter: MitoCheck Project Group,
Jean-Karim Hériché: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
Jutta Bulkescher: MitoCheck Project Group,
Holger Erfle: MitoCheck Project Group,
Christian Conrad: MitoCheck Project Group,
Phill Rogers: MitoCheck Project Group,
Ina Poser: Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
Michael Held: MitoCheck Project Group,
Urban Liebel: MitoCheck Project Group,
Cihan Cetin: Cell Biology/Biophysics Units, Structural and,
Frank Sieckmann: Leica Microsystems CMS GmbH, Am Friedensplatz 3, D-68165 Mannheim, Germany
Gregoire Pau: European Bioinformatics Institute, European Molecular Biology Laboratory
Rolf Kabbe: German Cancer Research Center, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany
Annelie Wünsche: Gene Expression and,
Venkata Satagopam: Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany
Michael H. A. Schmitz: Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland
Catherine Chapuis: Cell Biology/Biophysics Units, Structural and,
Daniel W. Gerlich: Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland
Reinhard Schneider: Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany
Roland Eils: German Cancer Research Center, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany
Wolfgang Huber: European Bioinformatics Institute, European Molecular Biology Laboratory
Jan-Michael Peters: Institute for Molecular Pathology, Dr Bohr Gasse 7, A-1030 Vienna, Austria
Anthony A. Hyman: Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
Richard Durbin: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
Rainer Pepperkok: Cell Biology/Biophysics Units, Structural and,
Jan Ellenberg: Gene Expression and,

Nature, 2010, vol. 464, issue 7289, 721-727

Abstract: Abstract Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the ∼21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.

Date: 2010
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DOI: 10.1038/nature08869

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