Structural mechanism of C-type inactivation in K+ channels

Luis G. Cuello, Vishwanath Jogini, D. Marien Cortes and Eduardo Perozo ()
Additional contact information
Luis G. Cuello: and Institute for Biophysical Dynamics, University of Chicago
Vishwanath Jogini: and Institute for Biophysical Dynamics, University of Chicago
D. Marien Cortes: and Institute for Biophysical Dynamics, University of Chicago
Eduardo Perozo: and Institute for Biophysical Dynamics, University of Chicago

Nature, 2010, vol. 466, issue 7303, 203-208

Abstract: Abstract Interconversion between conductive and non-conductive forms of the K+ channel selectivity filter underlies a variety of gating events, from flicker transitions (at the microsecond timescale) to C-type inactivation (millisecond to second timescale). Here we report the crystal structure of the Streptomyces lividans K+ channel KcsA in its open-inactivated conformation and investigate the mechanism of C-type inactivation gating at the selectivity filter from channels ‘trapped’ in a series of partially open conformations. Five conformer classes were identified with openings ranging from 12 Å in closed KcsA (Cα–Cα distances at Thr 112) to 32 Å when fully open. They revealed a remarkable correlation between the degree of gate opening and the conformation and ion occupancy of the selectivity filter. We show that a gradual filter backbone reorientation leads first to a loss of the S2 ion binding site and a subsequent loss of the S3 binding site, presumably abrogating ion conduction. These structures indicate a molecular basis for C-type inactivation in K+ channels.

Date: 2010
References: Add references at CitEc
Citations: View citations in EconPapers (9)

Downloads: (external link)
https://www.nature.com/articles/nature09153 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:466:y:2010:i:7303:d:10.1038_nature09153

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature09153

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().