Can controversies be put to REST?

Helle F. Jørgensen () and Amanda G. Fisher ()
Additional contact information
Helle F. Jørgensen: Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus
Amanda G. Fisher: Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus

Nature, 2010, vol. 467, issue 7311, E3-E4

Abstract: Abstract Arising from: S. K. Singh et al. Nature 453, 223–227 (2008)10.1038/nature06863 ; Singh et al. reply The contribution of REST to embryonic stem (ES) cell pluripotency has been uncertain. Two years ago, Singh et al.1 claimed that Rest+/− and REST knock-down ES cells expressed reduced levels of pluripotency markers1, in contrast to a prior2 and subsequent reports3,4,5,6. To understand the basis of this difference, we analysed the YHC334 (YHC) and RRC160 (RRC) gene-trap ES cell lines used by Singh et al.1, obtained directly from BayGenomics. Both REST mutant lines generated REST–βGeo fusion proteins, but expressed pluripotency genes at levels similar to appropriately matched parental wild ES cells, consistent with expression being REST–independent.

Date: 2010
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature09305 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:467:y:2010:i:7311:d:10.1038_nature09305

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature09305

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().