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Singh et al. reply

Sanjay K. Singh, Mohamedi N. Kagalwala, Jan Parker-Thornburg, Henry Adams and Sadhan Majumder
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Sanjay K. Singh: The University of Texas M. D. Anderson Cancer Center
Mohamedi N. Kagalwala: The University of Texas M. D. Anderson Cancer Center
Jan Parker-Thornburg: The University of Texas M. D. Anderson Cancer Center
Henry Adams: The University of Texas M. D. Anderson Cancer Center
Sadhan Majumder: The University of Texas M. D. Anderson Cancer Center

Nature, 2010, vol. 467, issue 7311, E5-E5

Abstract: Abstract Replying to: H. F. Jørgensen & A. G. Fisher Nature 467, doi:10.1038/nature09305 (2010) Jørgensen and Fisher 1 suggest that the discrepancy with our results2 could be attributable to our use of E14Tg2a (or its derivatives, such as OS25) rather than E14Tg2a.4 as the parental control line for the REST+/− cells (YHC and RRC). We have now reconfirmed our use of E14Tg2a.4 clonal cells as the control cells. Also, we have found that the YHC and RRC cells used in our experiments2, originally purchased from Bay Genomics, differ from the YHC and RRC cells used by Jørgensen and Fisher1 with respect to pluripotency-based on alkaline phosphatase/self-renewal assays (N. Song and S.K.S., unpublished results). We are currently using other assays to confirm these observations.

Date: 2010
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DOI: 10.1038/nature09306

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