Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions

Takuhiro Ito and Shigeyuki Yokoyama ()
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Takuhiro Ito: Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Shigeyuki Yokoyama: Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Nature, 2010, vol. 467, issue 7315, 612-616

Abstract: Enzymes sharing space on tRNA Synthesis of Glu-tRNA(Glu) and Gln-tRNA(Gln) begins with a common reaction, in which the glutamyl-tRNA synthetase (GluRS) glutamylates both tRNA(Glu) and tRNA(Gln). The Glu-tRNA(Gln) is then specifically converted to Gln-tRNA(Gln) by an amidotransferase. Takuhiro Ito and Shigeyuki Yokoyama have now solved the structure of a bacterial macromolecular 'glutamine transamidosome' complex containing tRNA(Gln), GluRS and the trimeric amidotransferase GatCAB. The structure explains the hand-off mechanism between the two enzymes, in which only one can assume a productive state at any time, and suggests that there is an intermediary state in which neither enzyme is productive.

Date: 2010
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DOI: 10.1038/nature09411

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