In vivo imaging of labelled endogenous β-actin mRNA during nucleocytoplasmic transport

David Grünwald and Robert H. Singer ()
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David Grünwald: Kavli Institute of NanoScience, TU Delft, Lorentzweg 1, 2628 CJ Delft, The Netherlands
Robert H. Singer: Albert Einstein College of Medicine

Nature, 2010, vol. 467, issue 7315, 604-607

Abstract: Nuclear pore complex selectivity Nuclear pore complexes selectively transport cargoes across the nuclear envelope. Now, two studies published in this issue follow the movement of single cargo proteins (Lowe et al.) and mRNA transcripts (David Grünwald and Robert Singer) as they translocate across the nuclear envelope, with nanometre precision and high temporal resolution. They discover previously unidentified transport sub-steps and provide fundamental insight into the mechanism of selective transport through the nuclear pore complexes.

Date: 2010
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DOI: 10.1038/nature09438

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