Jasmonate perception by inositol-phosphate-potentiated COI1–JAZ co-receptor

Laura B. Sheard, Xu Tan, Haibin Mao, John Withers, Gili Ben-Nissan, Thomas R. Hinds, Yuichi Kobayashi, Fong-Fu Hsu, Michal Sharon, John Browse, Sheng Yang He, Josep Rizo, Gregg A. Howe and Ning Zheng ()
Additional contact information
Laura B. Sheard: Box 357280, University of Washington
Xu Tan: Box 357280, University of Washington
Haibin Mao: Box 357280, University of Washington
John Withers: Michigan State University
Gili Ben-Nissan: Weizmann Institute of Science
Thomas R. Hinds: Box 357280, University of Washington
Yuichi Kobayashi: Tokyo Institute of Technology, 4259-B52 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan
Fong-Fu Hsu: Mass Spectrometry Resource, Diabetes, Metabolism, and Lipid research, Washington University School of Medicine
Michal Sharon: Weizmann Institute of Science
John Browse: Institute of Biological Chemistry, Washington State University
Sheng Yang He: Michigan State University
Josep Rizo: University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
Gregg A. Howe: Michigan State University
Ning Zheng: Box 357280, University of Washington

Nature, 2010, vol. 468, issue 7322, 400-405

Abstract: Abstract Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

Date: 2010
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DOI: 10.1038/nature09430

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