Structure and control of the actin regulatory WAVE complex

Zhucheng Chen, Dominika Borek, Shae B. Padrick, Timothy S. Gomez, Zoltan Metlagel, Ayman M. Ismail, Junko Umetani, Daniel D. Billadeau, Zbyszek Otwinowski and Michael K. Rosen ()
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Zhucheng Chen: University of Texas Southwestern Medical Center at Dallas
Dominika Borek: University of Texas Southwestern Medical Center at Dallas
Shae B. Padrick: University of Texas Southwestern Medical Center at Dallas
Timothy S. Gomez: Schulze Center for Novel Therapeutics, College of Medicine, Mayo Clinic
Zoltan Metlagel: University of Texas Southwestern Medical Center at Dallas
Ayman M. Ismail: University of Texas Southwestern Medical Center at Dallas
Junko Umetani: University of Texas Southwestern Medical Center at Dallas
Daniel D. Billadeau: Schulze Center for Novel Therapeutics, College of Medicine, Mayo Clinic
Zbyszek Otwinowski: University of Texas Southwestern Medical Center at Dallas
Michael K. Rosen: University of Texas Southwestern Medical Center at Dallas

Nature, 2010, vol. 468, issue 7323, 533-538

Abstract: Abstract Members of the Wiskott–Aldrich syndrome protein (WASP) family control cytoskeletal dynamics by promoting actin filament nucleation with the Arp2/3 complex. The WASP relative WAVE regulates lamellipodia formation within a 400-kilodalton, hetero-pentameric WAVE regulatory complex (WRC). The WRC is inactive towards the Arp2/3 complex, but can be stimulated by the Rac GTPase, kinases and phosphatidylinositols. Here we report the 2.3-ångstrom crystal structure of the WRC and complementary mechanistic analyses. The structure shows that the activity-bearing VCA motif of WAVE is sequestered by a combination of intramolecular and intermolecular contacts within the WRC. Rac and kinases appear to destabilize a WRC element that is necessary for VCA sequestration, suggesting the way in which these signals stimulate WRC activity towards the Arp2/3 complex. The spatial proximity of the Rac binding site and the large basic surface of the WRC suggests how the GTPase and phospholipids could cooperatively recruit the complex to membranes.

Date: 2010
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DOI: 10.1038/nature09623

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