U1 snRNP protects pre-mRNAs from premature cleavage and polyadenylation

Daisuke Kaida, Michael G. Berg, Ihab Younis, Mumtaz Kasim, Larry N. Singh, Lili Wan and Gideon Dreyfuss ()
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Daisuke Kaida: Howard Hughes Medical Institute, University of Pennsylvania School of Medicine
Michael G. Berg: Howard Hughes Medical Institute, University of Pennsylvania School of Medicine
Ihab Younis: Howard Hughes Medical Institute, University of Pennsylvania School of Medicine
Mumtaz Kasim: Howard Hughes Medical Institute, University of Pennsylvania School of Medicine
Larry N. Singh: Howard Hughes Medical Institute, University of Pennsylvania School of Medicine
Lili Wan: Howard Hughes Medical Institute, University of Pennsylvania School of Medicine
Gideon Dreyfuss: Howard Hughes Medical Institute, University of Pennsylvania School of Medicine

Nature, 2010, vol. 468, issue 7324, 664-668

Abstract: Abstract In eukaryotes, U1 small nuclear ribonucleoprotein (snRNP) forms spliceosomes in equal stoichiometry with U2, U4, U5 and U6 snRNPs; however, its abundance in human far exceeds that of the other snRNPs. Here we used antisense morpholino oligonucleotide to U1 snRNA to achieve functional U1 snRNP knockdown in HeLa cells, and identified accumulated unspliced pre-mRNAs by genomic tiling microarrays. In addition to inhibiting splicing, U1 snRNP knockdown caused premature cleavage and polyadenylation in numerous pre-mRNAs at cryptic polyadenylation signals, frequently in introns near (

Date: 2010
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DOI: 10.1038/nature09479

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