Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites

Andreas H. Ratje, Justus Loerke, Aleksandra Mikolajka, Matthias Brünner, Peter W. Hildebrand, Agata L. Starosta, Alexandra Dönhöfer, Sean R. Connell, Paola Fucini, Thorsten Mielke, Paul C. Whitford, José N. Onuchic, Yanan Yu, Karissa Y. Sanbonmatsu, Roland K. Hartmann, Pawel A. Penczek, Daniel N. Wilson () and Christian M. T. Spahn ()
Additional contact information
Andreas H. Ratje: Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5–9, 10117-Berlin, Germany
Justus Loerke: Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5–9, 10117-Berlin, Germany
Aleksandra Mikolajka: Ludwig-Maximilians-Universität, Feodor-Lynenstrasse 25, 81377 München, Germany
Matthias Brünner: Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5–9, 10117-Berlin, Germany
Peter W. Hildebrand: Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5–9, 10117-Berlin, Germany
Agata L. Starosta: Ludwig-Maximilians-Universität, Feodor-Lynenstrasse 25, 81377 München, Germany
Alexandra Dönhöfer: Ludwig-Maximilians-Universität, Feodor-Lynenstrasse 25, 81377 München, Germany
Sean R. Connell: Frankfurt Institute for Molecular Life Sciences, Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Max-von Laue-Strasse 7, D-60438 Frankfurt am Main, Germany
Paola Fucini: Frankfurt Institute for Molecular Life Sciences, Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Max-von Laue-Strasse 7, D-60438 Frankfurt am Main, Germany
Thorsten Mielke: Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5–9, 10117-Berlin, Germany
Paul C. Whitford: Theoretical Biology and Biophysics Group, Los Alamos National Laboratory
José N. Onuchic: University of California
Yanan Yu: Florida State University
Karissa Y. Sanbonmatsu: Theoretical Biology and Biophysics Group, Los Alamos National Laboratory
Roland K. Hartmann: Institut für Pharmazeutische Chemie, Philipps-Universität Marburg
Pawel A. Penczek: The University of Texas – Houston Medical School, 6431 Fannin
Daniel N. Wilson: Ludwig-Maximilians-Universität, Feodor-Lynenstrasse 25, 81377 München, Germany
Christian M. T. Spahn: Institut für Medizinische Physik und Biophysik, Charite – Universitätsmedizin Berlin, Ziegelstrasse 5–9, 10117-Berlin, Germany

Nature, 2010, vol. 468, issue 7324, 713-716

Abstract: Turning heads on tRNA translocation on the ribosome During translation, transfer RNAs enter the ribosome and then move sequentially through three sites, known as A, P and E, as they transfer their attached amino acids onto the growing peptide chain. How the ribosome facilitates tRNA translocation between the sites remains largely unknown. Christian Spahn and colleagues have used multiparticle cryoelectron microscopy of a ribosome bound to the translation elongation factor, EF-G, to get information about tRNA movement. They identify two new sub-states and conclude that, following spontaneous inter-subunit ratcheting, translocation is the direct result of head swivelling and unratcheting of the 30S ribosomal subunit.

Date: 2010
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DOI: 10.1038/nature09547

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