Single mimivirus particles intercepted and imaged with an X-ray laser

M. Marvin Seibert, Tomas Ekeberg, Filipe R. N. C. Maia, Martin Svenda, Jakob Andreasson, Olof Jönsson, Duško Odić, Bianca Iwan, Andrea Rocker, Daniel Westphal, Max Hantke, Daniel P. DePonte, Anton Barty, Joachim Schulz, Lars Gumprecht, Nicola Coppola, Andrew Aquila, Mengning Liang, Thomas A. White, Andrew Martin, Carl Caleman, Stephan Stern, Chantal Abergel (), Virginie Seltzer, Jean-Michel Claverie, Christoph Bostedt, John D. Bozek, Sébastien Boutet, A. Alan Miahnahri, Marc Messerschmidt, Jacek Krzywinski, Garth Williams, Keith O. Hodgson, Michael J. Bogan, Christina Y. Hampton, Raymond G. Sierra, Dmitri Starodub, Inger Andersson, Saša Bajt, Miriam Barthelmess, John C. H. Spence, Petra Fromme, Uwe Weierstall, Richard Kirian, Mark Hunter, R. Bruce Doak, Stefano Marchesini, Stefan P. Hau-Riege, Matthias Frank, Robert L. Shoeman, Lukas Lomb, Sascha W. Epp, Robert Hartmann, Daniel Rolles, Artem Rudenko, Carlo Schmidt, Lutz Foucar, Nils Kimmel, Peter Holl, Benedikt Rudek, Benjamin Erk, André Hömke, Christian Reich, Daniel Pietschner, Georg Weidenspointner, Lothar Strüder, Günter Hauser, Hubert Gorke, Joachim Ullrich, Ilme Schlichting, Sven Herrmann, Gerhard Schaller, Florian Schopper, Heike Soltau, Kai-Uwe Kühnel, Robert Andritschke, Claus-Dieter Schröter, Faton Krasniqi, Mario Bott, Sebastian Schorb, Daniela Rupp, Marcus Adolph, Tais Gorkhover, Helmut Hirsemann, Guillaume Potdevin, Heinz Graafsma, Björn Nilsson, Henry N. Chapman and Janos Hajdu ()
Additional contact information
M. Marvin Seibert: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Tomas Ekeberg: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Filipe R. N. C. Maia: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Martin Svenda: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Jakob Andreasson: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Olof Jönsson: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Duško Odić: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Bianca Iwan: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Andrea Rocker: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Daniel Westphal: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Max Hantke: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Daniel P. DePonte: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Anton Barty: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Joachim Schulz: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Lars Gumprecht: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Nicola Coppola: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Andrew Aquila: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Mengning Liang: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Thomas A. White: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Andrew Martin: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Carl Caleman: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Stephan Stern: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Chantal Abergel: Information Génomique et Structurale, CNRS-UPR2589, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France
Virginie Seltzer: Information Génomique et Structurale, CNRS-UPR2589, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France
Jean-Michel Claverie: Information Génomique et Structurale, CNRS-UPR2589, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France
Christoph Bostedt: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
John D. Bozek: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Sébastien Boutet: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
A. Alan Miahnahri: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Marc Messerschmidt: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Jacek Krzywinski: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Garth Williams: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Keith O. Hodgson: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Michael J. Bogan: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Christina Y. Hampton: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Raymond G. Sierra: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Dmitri Starodub: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Inger Andersson: Swedish University of Agricultural Sciences, Uppsala Biomedical Centre, Box 590, S-751 24 Uppsala, Sweden
Saša Bajt: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Miriam Barthelmess: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
John C. H. Spence: PSF470, Arizona State University
Petra Fromme: Arizona State University
Uwe Weierstall: PSF470, Arizona State University
Richard Kirian: PSF470, Arizona State University
Mark Hunter: Arizona State University
R. Bruce Doak: PSF470, Arizona State University
Stefano Marchesini: Advanced Light Source, Lawrence Berkeley National Laboratory
Stefan P. Hau-Riege: Lawrence Livermore National Laboratory, 7000 East Avenue, Mail Stop L-211, Livermore, California 94551, USA
Matthias Frank: Lawrence Livermore National Laboratory, 7000 East Avenue, Mail Stop L-211, Livermore, California 94551, USA
Robert L. Shoeman: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Lukas Lomb: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Sascha W. Epp: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Robert Hartmann: PNSensor GmbH, Römerstrasse 28, 80803 München, Germany
Daniel Rolles: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Artem Rudenko: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Carlo Schmidt: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Lutz Foucar: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Nils Kimmel: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Peter Holl: PNSensor GmbH, Römerstrasse 28, 80803 München, Germany
Benedikt Rudek: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Benjamin Erk: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
André Hömke: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Christian Reich: PNSensor GmbH, Römerstrasse 28, 80803 München, Germany
Daniel Pietschner: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Georg Weidenspointner: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Lothar Strüder: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Günter Hauser: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Hubert Gorke: Forschungszentrum Jülich, Institut ZEL, 52425 Jülich, Germany
Joachim Ullrich: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Ilme Schlichting: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Sven Herrmann: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Gerhard Schaller: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Florian Schopper: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Heike Soltau: PNSensor GmbH, Römerstrasse 28, 80803 München, Germany
Kai-Uwe Kühnel: Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany
Robert Andritschke: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Claus-Dieter Schröter: Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany
Faton Krasniqi: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Mario Bott: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Sebastian Schorb: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Daniela Rupp: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Marcus Adolph: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Tais Gorkhover: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Helmut Hirsemann: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Guillaume Potdevin: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Heinz Graafsma: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Björn Nilsson: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Henry N. Chapman: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Janos Hajdu: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden

Nature, 2011, vol. 470, issue 7332, 78-81

Abstract: Biological imaging with the LCLS X-ray laser The start-up of the Linac Coherent Light Source (LCLS), the new femtosecond hard X-ray laser facility in Stanford, California, has brought high expectations of a new era for biological imaging. The intense, ultrashort X-ray pulses allow diffraction imaging of small structures before radiation damage occurs. Two papers in this issue of Nature present proof-of-concept experiments showing the LCLS in action. Chapman et al. tackle structure determination from nanocrystals of macromolecules that cannot be grown in large crystals. They obtain more than three million diffraction patterns from a stream of nanocrystals of the membrane protein photosystem I, and assemble a three-dimensional data set for this protein. Seibert et al. obtain images of a non-crystalline biological sample, mimivirus, by injecting a beam of cooled mimivirus particles into the X-ray beam.

Date: 2011
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DOI: 10.1038/nature09748

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