Femtosecond X-ray protein nanocrystallography

Henry N. Chapman (), Petra Fromme, Anton Barty, Thomas A. White, Richard A. Kirian, Andrew Aquila, Mark S. Hunter, Joachim Schulz, Daniel P. DePonte, Uwe Weierstall, R. Bruce Doak, Filipe R. N. C. Maia, Andrew V. Martin, Ilme Schlichting, Lukas Lomb, Nicola Coppola, Robert L. Shoeman, Sascha W. Epp, Robert Hartmann, Daniel Rolles, Artem Rudenko, Lutz Foucar, Nils Kimmel, Georg Weidenspointner, Peter Holl, Mengning Liang, Miriam Barthelmess, Carl Caleman, Sébastien Boutet, Michael J. Bogan, Jacek Krzywinski, Christoph Bostedt, Saša Bajt, Lars Gumprecht, Benedikt Rudek, Benjamin Erk, Carlo Schmidt, André Hömke, Christian Reich, Daniel Pietschner, Lothar Strüder, Günter Hauser, Hubert Gorke, Joachim Ullrich, Sven Herrmann, Gerhard Schaller, Florian Schopper, Heike Soltau, Kai-Uwe Kühnel, Marc Messerschmidt, John D. Bozek, Stefan P. Hau-Riege, Matthias Frank, Christina Y. Hampton, Raymond G. Sierra, Dmitri Starodub, Garth J. Williams, Janos Hajdu, Nicusor Timneanu, M. Marvin Seibert, Jakob Andreasson, Andrea Rocker, Olof Jönsson, Martin Svenda, Stephan Stern, Karol Nass, Robert Andritschke, Claus-Dieter Schröter, Faton Krasniqi, Mario Bott, Kevin E. Schmidt, Xiaoyu Wang, Ingo Grotjohann, James M. Holton, Thomas R. M. Barends, Richard Neutze, Stefano Marchesini, Raimund Fromme, Sebastian Schorb, Daniela Rupp, Marcus Adolph, Tais Gorkhover, Inger Andersson, Helmut Hirsemann, Guillaume Potdevin, Heinz Graafsma, Björn Nilsson and John C. H. Spence
Additional contact information
Henry N. Chapman: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Petra Fromme: Arizona State University
Anton Barty: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Thomas A. White: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Richard A. Kirian: Arizona State University
Andrew Aquila: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Mark S. Hunter: Arizona State University
Joachim Schulz: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Daniel P. DePonte: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Uwe Weierstall: Arizona State University
R. Bruce Doak: Arizona State University
Filipe R. N. C. Maia: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Andrew V. Martin: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Ilme Schlichting: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Lukas Lomb: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Nicola Coppola: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Robert L. Shoeman: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Sascha W. Epp: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Robert Hartmann: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany
Daniel Rolles: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Artem Rudenko: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Lutz Foucar: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Nils Kimmel: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Georg Weidenspointner: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Peter Holl: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany
Mengning Liang: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Miriam Barthelmess: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Carl Caleman: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Sébastien Boutet: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Michael J. Bogan: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Jacek Krzywinski: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Christoph Bostedt: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Saša Bajt: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Lars Gumprecht: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Benedikt Rudek: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Benjamin Erk: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Carlo Schmidt: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
André Hömke: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Christian Reich: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany
Daniel Pietschner: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Lothar Strüder: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Günter Hauser: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Hubert Gorke: Forschungszentrum Jülich, Institut ZEL, 52425 Jülich, Germany
Joachim Ullrich: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Sven Herrmann: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Gerhard Schaller: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Florian Schopper: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Heike Soltau: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany
Kai-Uwe Kühnel: Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany
Marc Messerschmidt: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
John D. Bozek: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Stefan P. Hau-Riege: Lawrence Livermore National Laboratory, 7000 East Avenue, Mail Stop L-211, Livermore, California 94551, USA
Matthias Frank: Lawrence Livermore National Laboratory, 7000 East Avenue, Mail Stop L-211, Livermore, California 94551, USA
Christina Y. Hampton: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Raymond G. Sierra: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Dmitri Starodub: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Garth J. Williams: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA
Janos Hajdu: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Nicusor Timneanu: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
M. Marvin Seibert: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Jakob Andreasson: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Andrea Rocker: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Olof Jönsson: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Martin Svenda: Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
Stephan Stern: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Karol Nass: University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
Robert Andritschke: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
Claus-Dieter Schröter: Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany
Faton Krasniqi: Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
Mario Bott: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Kevin E. Schmidt: Arizona State University
Xiaoyu Wang: Arizona State University
Ingo Grotjohann: Arizona State University
James M. Holton: Advanced Light Source, Lawrence Berkeley National Laboratory
Thomas R. M. Barends: Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany
Richard Neutze: Biochemistry and Biophysics, University of Gothenburg, SE-405 30 Gothenburg, Sweden
Stefano Marchesini: Advanced Light Source, Lawrence Berkeley National Laboratory
Raimund Fromme: Arizona State University
Sebastian Schorb: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Daniela Rupp: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Marcus Adolph: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Tais Gorkhover: Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany
Inger Andersson: Swedish University of Agricultural Sciences, Uppsala Biomedical Centre, Box 590, S-751 24 Uppsala, Sweden
Helmut Hirsemann: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Guillaume Potdevin: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Heinz Graafsma: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
Björn Nilsson: Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
John C. H. Spence: Arizona State University

Nature, 2011, vol. 470, issue 7332, 73-77

Abstract: Biological imaging with the LCLS X-ray laser The start-up of the Linac Coherent Light Source (LCLS), the new femtosecond hard X-ray laser facility in Stanford, California, has brought high expectations of a new era for biological imaging. The intense, ultrashort X-ray pulses allow diffraction imaging of small structures before radiation damage occurs. Two papers in this issue of Nature present proof-of-concept experiments showing the LCLS in action. Chapman et al. tackle structure determination from nanocrystals of macromolecules that cannot be grown in large crystals. They obtain more than three million diffraction patterns from a stream of nanocrystals of the membrane protein photosystem I, and assemble a three-dimensional data set for this protein. Seibert et al. obtain images of a non-crystalline biological sample, mimivirus, by injecting a beam of cooled mimivirus particles into the X-ray beam.

Date: 2011
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DOI: 10.1038/nature09750

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