Central dogma at the single-molecule level in living cells

Gene-Wei Li and X. Sunney Xie ()
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Gene-Wei Li: Harvard University
X. Sunney Xie: Harvard University

Nature, 2011, vol. 475, issue 7356, 308-315

Abstract: Abstract Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time. This leads to heterogeneity in the messenger-RNA and protein copy numbers in a population of cells with identical genomes. With advanced single-cell fluorescence microscopy, it is now possible to quantify transcriptomes and proteomes with single-molecule sensitivity. Dynamic processes such as transcription-factor binding, transcription and translation can be monitored in real time, providing quantitative descriptions of the central dogma of molecular biology and the demonstration that a stochastic single-molecule event can determine the phenotype of a cell.

Date: 2011
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DOI: 10.1038/nature10315

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