Crystal structure of the human centromeric nucleosome containing CENP-A

Hiroaki Tachiwana, Wataru Kagawa, Tatsuya Shiga, Akihisa Osakabe, Yuta Miya, Kengo Saito, Yoko Hayashi-Takanaka, Takashi Oda, Mamoru Sato, Sam-Yong Park, Hiroshi Kimura and Hitoshi Kurumizaka ()
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Hiroaki Tachiwana: Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Wataru Kagawa: Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Tatsuya Shiga: Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Akihisa Osakabe: Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Yuta Miya: Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Kengo Saito: Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Yoko Hayashi-Takanaka: Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan
Takashi Oda: Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan
Mamoru Sato: Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan
Sam-Yong Park: Protein Design Laboratory, Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan
Hiroshi Kimura: Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan
Hitoshi Kurumizaka: Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan

Nature, 2011, vol. 476, issue 7359, 232-235

Abstract: The centromeric nucleosome Centromeres are epigenetically marked by the assembly of nucleosomes containing CENP-A, a centromere-specific histone H3 variant. Tachiwana et al. report the crystal structure of the human centromeric nucleosome bound to DNA. They find a canonical arrangement of an octamer of histone proteins with DNA wrapped in a left-handed superhelix. There is flexibility in the DNA regions at the entrance and exit of the nucleosome, and a loop in CENP-A may help to stabilize its incorporation into centromeric chromatin. As the first view of the CENP-A-containing nucleosome, the structure helps to clarify various models that have been debated in the literature.

Date: 2011
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DOI: 10.1038/nature10258

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