In vitro centromere and kinetochore assembly on defined chromatin templates

Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller and Aaron F. Straight ()
Additional contact information
Annika Guse: Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA
Christopher W. Carroll: Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA
Ben Moree: Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA
Colin J. Fuller: Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA
Aaron F. Straight: Stanford Medical School, Beckman 409A, Stanford, California 94305-5307, USA

Nature, 2011, vol. 477, issue 7364, 354-358

Abstract: In vitro reconstitution of the centromere Centromeres are specialized chromatin domains that are essential for chromosome segregation and cell division, but it is unclear how they are established and assembled to generate microtubule-binding sites, or kinetochores, during mitosis. Here, Aaron Straight and colleagues have reconstituted vertebrate centromeric chromatin in vitro and show that, when added to cell-free extracts, this reconstituted CENP-A chromatin is sufficient for the assembly of centromere and kinetochore proteins, binding of microtubules and mitotic checkpoint function. This cell-free system is used to dissect various molecular features of CENP-A, and should be a valuable approach to study the complex centromeres of vertebrates.

Date: 2011
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/nature10379 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:477:y:2011:i:7364:d:10.1038_nature10379

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature10379

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().