Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

Jessica S. Dymond, Sarah M. Richardson, Candice E. Coombes, Timothy Babatz, Héloïse Muller, Narayana Annaluru, William J. Blake, Joy W. Schwerzmann, Junbiao Dai, Derek L. Lindstrom, Annabel C. Boeke, Daniel E. Gottschling, Srinivasan Chandrasegaran, Joel S. Bader and Jef D. Boeke ()
Additional contact information
Jessica S. Dymond: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
Sarah M. Richardson: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
Candice E. Coombes: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
Timothy Babatz: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
Héloïse Muller: Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
Narayana Annaluru: Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
William J. Blake: Codon Devices, 99 Erie Street, Cambridge, Massachusetts 02139, USA
Joy W. Schwerzmann: Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
Junbiao Dai: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
Derek L. Lindstrom: Fred Hutchinson Cancer Research Center, Mailstop A3-025, PO Box 19024, Seattle, Washington 98109, USA
Annabel C. Boeke: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
Daniel E. Gottschling: Fred Hutchinson Cancer Research Center, Mailstop A3-025, PO Box 19024, Seattle, Washington 98109, USA
Srinivasan Chandrasegaran: Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
Joel S. Bader: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
Jef D. Boeke: High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA

Nature, 2011, vol. 477, issue 7365, 471-476

Abstract: A part-synthetic yeast genome A milestone in biology was reached in 2010, with the production of a viable bacterium with a genome that had been reassembled artificially from synthetic DNA segments. Now Jef Boeke and colleagues report the production of the world's first synthetic eukaryotic chromosome arms, a first step in a project called Sc2.0, which aims to design and construct an entirely synthetic version of the Saccharomyces cerevisiae genome. In this initial phase, two custom-designed synthetic chromosome arms were incorporated into the yeast genome, replacing the endogenous sequence.

Date: 2011
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DOI: 10.1038/nature10403

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