Human oocytes reprogram somatic cells to a pluripotent state

Scott Noggle, Ho-Lim Fung, Athurva Gore, Hector Martinez, Kathleen Crumm Satriani, Robert Prosser, Kiboong Oum, Daniel Paull, Sarah Druckenmiller, Matthew Freeby, Ellen Greenberg, Kun Zhang, Robin Goland, Mark V. Sauer, Rudolph L. Leibel and Dieter Egli ()
Additional contact information
Scott Noggle: The New York Stem Cell Foundation Laboratory
Ho-Lim Fung: University of California at San Diego
Athurva Gore: University of California at San Diego
Hector Martinez: The New York Stem Cell Foundation Laboratory
Kathleen Crumm Satriani: Center for Women’s Reproductive Care, College of Physicians and Surgeons, Columbia University
Robert Prosser: Center for Women’s Reproductive Care, College of Physicians and Surgeons, Columbia University
Kiboong Oum: Center for Women’s Reproductive Care, College of Physicians and Surgeons, Columbia University
Daniel Paull: The New York Stem Cell Foundation Laboratory
Sarah Druckenmiller: The New York Stem Cell Foundation Laboratory
Matthew Freeby: Naomi Berrie Diabetes Center, College of Physicians and Surgeons, Columbia University
Ellen Greenberg: Naomi Berrie Diabetes Center, College of Physicians and Surgeons, Columbia University
Kun Zhang: University of California at San Diego
Robin Goland: Naomi Berrie Diabetes Center, College of Physicians and Surgeons, Columbia University
Mark V. Sauer: Center for Women’s Reproductive Care, College of Physicians and Surgeons, Columbia University
Rudolph L. Leibel: Naomi Berrie Diabetes Center, College of Physicians and Surgeons, Columbia University
Dieter Egli: The New York Stem Cell Foundation Laboratory

Nature, 2011, vol. 478, issue 7367, 70-75

Abstract: Abstract The exchange of the oocyte’s genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cells affected in degenerative human diseases. Such cells, carrying the patient’s genome, might be useful for cell replacement. Here we report that the development of human oocytes after genome exchange arrests at late cleavage stages in association with transcriptional abnormalities. In contrast, if the oocyte genome is not removed and the somatic cell genome is merely added, the resultant triploid cells develop to the blastocyst stage. Stem cell lines derived from these blastocysts differentiate into cell types of all three germ layers, and a pluripotent gene expression program is established on the genome derived from the somatic cell. This result demonstrates the feasibility of reprogramming human cells using oocytes and identifies removal of the oocyte genome as the primary cause of developmental failure after genome exchange.

Date: 2011
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DOI: 10.1038/nature10397

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