Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1

Valerie Garcia, Sarah E. L. Phelps, Stephen Gray and Matthew J. Neale ()
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Valerie Garcia: Genome Damage and Stability Centre, The University of Sussex
Sarah E. L. Phelps: Genome Damage and Stability Centre, The University of Sussex
Stephen Gray: Genome Damage and Stability Centre, The University of Sussex
Matthew J. Neale: Genome Damage and Stability Centre, The University of Sussex

Nature, 2011, vol. 479, issue 7372, 241-244

Abstract: DNA strand-break removal by Mre11 and Exo1 Specific DNA double-strand breaks are made during meiosis by Spo11, which remains bound to the DNA ends. Mre11 is a nuclease that can act exonucleolytically at DNA ends and endonucleolytically at internal sites. Previous studies have defined a role for the endonuclease, but not exonuclease, activity in DNA repair. Matthew Neale and colleagues show that Mre11 first makes a nick 300 bases from the end of the 5' strand, after which Mre11 degrades the DNA towards the break. Meanwhile, a second nuclease, Exo1, degrades the same strand in the opposite direction. This demonstrates that exonucleases can be loaded when the DNA end that is usually required for their initial binding is blocked.

Date: 2011
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DOI: 10.1038/nature10515

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