Structure of the immature retroviral capsid at 8 Å resolution by cryo-electron microscopy

Tanmay A. M. Bharat, Norman E. Davey, Pavel Ulbrich, James D. Riches, Alex de Marco, Michaela Rumlova, Carsten Sachse, Tomas Ruml and John A. G. Briggs ()
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Tanmay A. M. Bharat: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Norman E. Davey: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Pavel Ulbrich: Institute of Chemical Technology, Prague, Technicka 3, 166 28 Prague, Czech Republic
James D. Riches: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Alex de Marco: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Michaela Rumlova: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of Czech Republic, v.v.i., Flemingovo nám. 2, 166 10 Prague, Czech Republic
Carsten Sachse: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Tomas Ruml: Institute of Chemical Technology, Prague, Technicka 3, 166 28 Prague, Czech Republic
John A. G. Briggs: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany

Nature, 2012, vol. 487, issue 7407, 385-389

Abstract: A hybrid cryo-electron microscopy/tomography approach is used to solve the structure of the immature Mason–Pfizer monkey virus Gag lattice at a resolution of 8 Å, allowing the derivation of a model for the structure of retroviral capsid in the immature Gag shell.

Date: 2012
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DOI: 10.1038/nature11169

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