A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis

Paola Picotti (), Mathieu Clément-Ziza, Henry Lam, David S. Campbell, Alexander Schmidt, Eric W. Deutsch, Hannes Röst, Zhi Sun, Oliver Rinner, Lukas Reiter, Qin Shen, Jacob J. Michaelson, Andreas Frei, Simon Alberti, Ulrike Kusebauch, Bernd Wollscheid, Robert L. Moritz, Andreas Beyer () and Ruedi Aebersold ()
Additional contact information
Paola Picotti: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Mathieu Clément-Ziza: Biotechnology Centre, Technische Universitaet Dresden, D-01069 Dresden, Germany
Henry Lam: The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
David S. Campbell: Institute for Systems Biology
Alexander Schmidt: Proteomics Core Facility Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland
Eric W. Deutsch: Institute for Systems Biology
Hannes Röst: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Zhi Sun: Institute for Systems Biology
Oliver Rinner: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Lukas Reiter: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Qin Shen: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Jacob J. Michaelson: Biotechnology Centre, Technische Universitaet Dresden, D-01069 Dresden, Germany
Andreas Frei: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Simon Alberti: Max Planck Institute of Molecular Cell Biology and Genetics, D-01307 Dresden, Germany
Ulrike Kusebauch: Institute for Systems Biology
Bernd Wollscheid: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Robert L. Moritz: Institute for Systems Biology
Andreas Beyer: Biotechnology Centre, Technische Universitaet Dresden, D-01069 Dresden, Germany
Ruedi Aebersold: Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland

Nature, 2013, vol. 494, issue 7436, 266-270

Abstract: High-throughput peptide synthesis and mass spectrometry are used to generate a near-complete reference map of the Saccharomyces cerevisiae proteome; two versions of the map (supporting discovery- and hypothesis-driven proteomics) are then applied to a protein-based quantitative trait locus analysis.

Date: 2013
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DOI: 10.1038/nature11835

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