High-resolution analysis with novel cell-surface markers identifies routes to iPS cells

James O’Malley, Stavroula Skylaki, Kumiko A. Iwabuchi, Eleni Chantzoura, Tyson Ruetz, Anna Johnsson, Simon R. Tomlinson, Sten Linnarsson and Keisuke Kaji ()
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James O’Malley: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK
Stavroula Skylaki: Stem Cell Dynamics Research Unit, Helmholtz Center Munich, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
Kumiko A. Iwabuchi: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK
Eleni Chantzoura: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK
Tyson Ruetz: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK
Anna Johnsson: Laboratory for Molecular Neurobiology, Karolinska Institute, Scheeles väg 1, SE-171 77 Stockholm, Sweden
Simon R. Tomlinson: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK
Sten Linnarsson: Laboratory for Molecular Neurobiology, Karolinska Institute, Scheeles väg 1, SE-171 77 Stockholm, Sweden
Keisuke Kaji: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UK

Nature, 2013, vol. 499, issue 7456, 88-91

Abstract: Cellular reprogramming is shown to occur in an ordered, stepwise manner, marked by changes in the cell-surface markers CD44, ICAM1 and Nanog–eGFP; molecular characterization of discrete subpopulations of partially reprogrammed cells shows that reprogramming is not simply the reversal of the normal development process.

Date: 2013
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DOI: 10.1038/nature12243

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