c-kit+ cells minimally contribute cardiomyocytes to the heart

Jop H. van Berlo, Onur Kanisicak, Marjorie Maillet, Ronald J. Vagnozzi, Jason Karch, Suh-Chin J. Lin, Ryan C. Middleton, Eduardo Marbán and Jeffery D. Molkentin ()
Additional contact information
Jop H. van Berlo: Cincinnati Children’s Hospital Medical Center
Onur Kanisicak: Cincinnati Children’s Hospital Medical Center
Marjorie Maillet: Cincinnati Children’s Hospital Medical Center
Ronald J. Vagnozzi: Cincinnati Children’s Hospital Medical Center
Jason Karch: Cincinnati Children’s Hospital Medical Center
Suh-Chin J. Lin: Cincinnati Children’s Hospital Medical Center
Ryan C. Middleton: Cedars-Sinai Heart Institute, 8700 Beverly Boulevard
Eduardo Marbán: Cedars-Sinai Heart Institute, 8700 Beverly Boulevard
Jeffery D. Molkentin: Cincinnati Children’s Hospital Medical Center

Nature, 2014, vol. 509, issue 7500, 337-341

Abstract: Abstract If and how the heart regenerates after an injury event is highly debated. c-kit-expressing cardiac progenitor cells have been reported as the primary source for generation of new myocardium after injury. Here we generated two genetic approaches in mice to examine whether endogenous c-kit+ cells contribute differentiated cardiomyocytes to the heart during development, with ageing or after injury in adulthood. A complementary DNA encoding either Cre recombinase or a tamoxifen-inducible MerCreMer chimaeric protein was targeted to the Kit locus in mice and then bred with reporter lines to permanently mark cell lineage. Endogenous c-kit+ cells did produce new cardiomyocytes within the heart, although at a percentage of approximately 0.03 or less, and if a preponderance towards cellular fusion is considered, the percentage falls to below approximately 0.008. By contrast, c-kit+ cells amply generated cardiac endothelial cells. Thus, endogenous c-kit+ cells can generate cardiomyocytes within the heart, although probably at a functionally insignificant level.

Date: 2014
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/nature13309 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:509:y:2014:i:7500:d:10.1038_nature13309

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature13309

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().