High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

Yuexin Zhou, Shiyou Zhu, Changzu Cai, Pengfei Yuan, Chunmei Li, Yanyi Huang and Wensheng Wei ()
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Yuexin Zhou: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University
Shiyou Zhu: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University
Changzu Cai: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University
Pengfei Yuan: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University
Chunmei Li: Biodynamic Optical Imaging Centre (BIOPIC), College of Engineering, Peking University
Yanyi Huang: Biodynamic Optical Imaging Centre (BIOPIC), College of Engineering, Peking University
Wensheng Wei: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University

Nature, 2014, vol. 509, issue 7501, 487-491

Abstract: This study describes the construction of a focused CRISPR/Cas-based lentiviral library in human cells and a method of gene identification based on functional screening and high-throughput sequencing analysis.

Date: 2014
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DOI: 10.1038/nature13166

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