Ribosomal frameshifting in the CCR5 mRNA is regulated by miRNAs and the NMD pathway

Ashton Trey Belew, Arturas Meskauskas, Sharmishtha Musalgaonkar, Vivek M. Advani, Sergey O. Sulima, Wojciech K. Kasprzak, Bruce A. Shapiro and Jonathan D. Dinman ()
Additional contact information
Ashton Trey Belew: University of Maryland, College Park
Arturas Meskauskas: University of Maryland, College Park
Sharmishtha Musalgaonkar: University of Maryland, College Park
Vivek M. Advani: University of Maryland, College Park
Sergey O. Sulima: University of Maryland, College Park
Wojciech K. Kasprzak: Basic Science Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research
Bruce A. Shapiro: Basic Research Laboratory, National Cancer Institute
Jonathan D. Dinman: University of Maryland, College Park

Nature, 2014, vol. 512, issue 7514, 265-269

Abstract: Abstract Programmed −1 ribosomal frameshift (−1 PRF) signals redirect translating ribosomes to slip back one base on messenger RNAs. Although well characterized in viruses, how these elements may regulate cellular gene expression is not understood. Here we describe a −1 PRF signal in the human mRNA encoding CCR5, the HIV-1 co-receptor. CCR5 mRNA-mediated −1 PRF is directed by an mRNA pseudoknot, and is stimulated by at least two microRNAs. Mapping the mRNA–miRNA interaction suggests that formation of a triplex RNA structure stimulates −1 PRF. A −1 PRF event on the CCR5 mRNA directs translating ribosomes to a premature termination codon, destabilizing it through the nonsense-mediated mRNA decay pathway. At least one additional mRNA decay pathway is also involved. Functional −1 PRF signals that seem to be regulated by miRNAs are also demonstrated in mRNAs encoding six other cytokine receptors, suggesting a novel mode through which immune responses may be fine-tuned in mammalian cells.

Date: 2014
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/nature13429 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:512:y:2014:i:7514:d:10.1038_nature13429

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature13429

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().