Saturation editing of genomic regions by multiplex homology-directed repair

Gregory M. Findlay (), Evan A. Boyle, Ronald J. Hause, Jason C. Klein and Jay Shendure ()
Additional contact information
Gregory M. Findlay: University of Washington
Evan A. Boyle: University of Washington
Ronald J. Hause: University of Washington
Jason C. Klein: University of Washington
Jay Shendure: University of Washington

Nature, 2014, vol. 513, issue 7516, 120-123

Abstract: The authors perform saturation mutagenesis of genomic regions in their native endogenous chromosomal context by using CRISPR/Cas9 RNA-guided cleavage and multiplex homology-directed repair; its utility is demonstrated by measuring the effects of hundreds to thousands of genomic edits to BRCA1 and DBR1 on splicing and cellular fitness, respectively.

Date: 2014
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/nature13695 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:513:y:2014:i:7516:d:10.1038_nature13695

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature13695

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().