Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

Carolin Anders, Ole Niewoehner, Alessia Duerst and Martin Jinek ()
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Carolin Anders: University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Ole Niewoehner: University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Alessia Duerst: University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Martin Jinek: University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland

Nature, 2014, vol. 513, issue 7519, 569-573

Abstract: Crystal structure of the RNA-guided endonuclease Cas9 bound to a guide RNA and a target DNA duplex reveals how base-specific recognition of a short motif known as PAM in the DNA target results in localized strand separation in the DNA immediately upstream of the PAM, allowing the target DNA strand to hybridize to the guide RNA.

Date: 2014
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DOI: 10.1038/nature13579

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