Crystal structure of a eukaryotic group II intron lariat

Aaron R. Robart, Russell T. Chan, Jessica K. Peters, Kanagalaghatta R. Rajashankar and Navtej Toor ()
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Aaron R. Robart: University of California, San Diego, La Jolla, California 92093, USA
Russell T. Chan: University of California, San Diego, La Jolla, California 92093, USA
Jessica K. Peters: University of California, San Diego, La Jolla, California 92093, USA
Kanagalaghatta R. Rajashankar: Cornell University, Argonne National Laboratory, Argonne, Illinois 60439, USA
Navtej Toor: University of California, San Diego, La Jolla, California 92093, USA

Nature, 2014, vol. 514, issue 7521, 193-197

Abstract: Abstract The formation of branched lariat RNA is an evolutionarily conserved feature of splicing reactions for both group II and spliceosomal introns. The lariat is important for the fidelity of 5′ splice-site selection and consists of a 2′-5′ phosphodiester bond between a bulged adenosine and the 5′ end of the intron. To gain insight into this ubiquitous intramolecular linkage, we determined the crystal structure of a eukaryotic group IIB intron in the lariat form at 3.7 Å. This revealed that two tandem tetraloop-receptor interactions, η–η′ and π–π′, place domain VI in the core to position the lariat bond in the post-catalytic state. On the basis of structural and biochemical data, we propose that π–π′ is a dynamic interaction that mediates the transition between the two steps of splicing, with η–η′ serving an ancillary role. The structure also reveals a four-magnesium-ion cluster involved in both catalysis and positioning of the 5′ end. Given the evolutionary relationship between group II and nuclear introns, it is likely that this active site configuration exists in the spliceosome as well.

Date: 2014
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DOI: 10.1038/nature13790

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